Preparation of SSCs Recipients in Sheep; Effects of Anticancer Drugs

Introduction: This in vitro investigation aimed to assess the impact of taxol, carboplatin, vinblastine (VBL), and vincristine (VCR) on ovine spermatogonial stem cells (SSCs). SSCs can be extracted from the testes of an animal and cultured in a laboratory to increase their quantity for transplantation into a recipient. Upon transplantation, these cells maintain their population and initiate sperm production in the recipient animal. Materials & Methods: Adequate preparation of the recipient is a crucial aspect of spermatogonial stem cell transplantation. Drawing upon the findings of in vitro investigations conducted on SSCs, a tripartite regimen comprising carboplatin, taxol, and VCR was administered to four-month-old male Shal lambs at varying concentrations. The in vivo experiments included the culture of SSCs, GFRαl and c-myc specific gene expression analysis, and histological analysis.Results: The results demonstrated that injection of the aforementioned combination (at concentrations of ۲.۰۶۳۲, ۰.۹۰۶, and ۰.۰۰۷۲ mg, respectively) beneath the scrotums of each testicle of Shal lambs resulted in a significant reduction in the SSCs population. The expression of GFRαl and c-myc genes, as specific markers of stem cells, was reduced considerably in double and quadruple concentrations of carboplatin + taxol + vincristine compared to other treatments.Conclusion: The findings suggest that a tripartite regimen consisting of carboplatin, taxol, and VCR may be suitable for preparing sheep recipients for SSCs transplantation, based on the results of this study, the use of a combination of carboplatin, taxol, and vincristine is recommended for the preparation of SSC transplant receptors in sheep. Injection of a combination of carboplatin, taxol, and vincristine, equivalent to twice the concentration recommended for cancer treatment by the drug manufacturer, under the Scrotum of each of the testicles of Shal lambs significantly reduces the cell population.

کلیدواژه ها:

Expression ، Ovine ، Spermatogonial stem cells (SSCs) ، transplantation ، Vincristine