Mito-TEMPO Protects Buck’s Semen Quality During the Cooling Process

Background: Membrane lipid peroxidation is one of the most significant chemical stresses in sperm. However, the utilization of antioxidants in semen diluent protects cells against oxidative damage, which could be a beneficial way to preserve semen quality during the storage process. Objectives: The current study aimed to assess the effect of Mito-TEMPO on quality parameters of buck semen during the cooling process. Methods: In this experiment, ۵ Saanen bucks were used, and their semen samples were collected twice a week. After semen collection and primary evaluation, semen samples were diluted and assigned into ۵ equal aliquots. Then, the samples were added to extenders containing ۰, ۱, ۱۰, ۱۰۰, and ۱۰۰۰ μM Mito-TEMPO (triphenylphosphonium chloride). The prepared samples were stored at ۵ °C for up to ۶۰ h. Chilled sperm motility, progressive motility (PM), viability, mitochondrial activity, membrane functionality, and malondialdehyde (MDA) concentration were analyzed during ۰, ۳۰, and ۶۰ h. Results: In results at time ۰, no difference was observed among groups, but at ۳۰ and ۶۰ h storage, higher (P≤۰.۰۵) sperm total motility (TM), PM, mitochondrial activity, membrane functionality, viability, and lower lipid peroxidation were observed in ۱۰ and ۱۰۰ μM Mito-TEMPO groups than the others.Conclusion: In conclusion, supplementation of buck cooling medium with Mito-TEMPO as a novel mitochondria-targeted antioxidant could be an impressive method to conserve buck’s semen quality during the cooling storage process.

کلیدواژه ها:

Buck ، chilling ، Mito-TEMPO ، Spermatozoa