Effects of Feed Restriction and Prebiotic Supplementation on Growth Performance, Immune Responses, Microbial Population, and Intestinal Morphology of Broiler Chickens

This study examined the effects of feed restriction and prebiotic supplementation on broiler chickens’ performance, immune response, gut microbiota, and intestinal morphology. A total of ۲۴۰ one-day-old male Ross ۳۰۸ broilers were randomly assigned to four treatments: control (C), feed restriction (FR), prebiotic supplementation (P), and a combination of prebiotic supplementation with feed restriction (FR+P). The prebiotic contained mannan-oligosaccharides (MOSs) and beta-glucans at ۰.۱% of the diet. Feed restriction was set at ۸۰% ad libitum intake during the second week. The findings indicated that prebiotic supplementation increased the feed intake and weight gain, particularly during the early growth phase (p< ۰.۰۵). Feed restriction resulted in low daily weight gain and impaired cellular immune response (p< ۰.۰۵); however, these detrimental effects were partially mitigated by prebiotic supplementation. The FR+P group treated at ۲۱ days had higher counts of Lactobacillus with lower coliform counts in the cecum (p< ۰.۰۵). Moreover, prebiotic supplementation improved intestinal morphology as indicated by an increase in villus height and crypt depth of the duodenum and jejunum with a significant effect in the FR+P and P groups (p< ۰.۰۵). The FR+P group recorded the highest villus height of the duodenum at ۲۱ days, whereas the highest jejunal villus height and duodenal crypt depth were observed in the P group at ۴۲ days (p< ۰.۰۵). The cell-mediated immunity, as determined by footpad swelling following the injection of PHA, was greatly enhanced in birds fed prebiotics compared to birds with feed restriction alone (p< ۰.۰۵). Overall, prebiotics, particularly with feed restriction, enhanced gut health, immune function, and intestinal morphology, suggesting their potential in broiler production.

کلیدواژه ها:

mannan-oligosaccharides ، Lactobacillus ، Coliforms ، villus height‒crypt depth ratio