Effect of Zinc Oxide Nanoparticles on Hepatic Ischemia-Reperfusion Injury: Role of miR-۱۲۵b Expression in Possible Underlying Mechanisms

Background: This study examined the protective effects of zinc oxide nanoparticles (ZnO-NPs) on hepatic ischemia-reperfusion injury (HIRI) and their possible underlying mechanisms. Methods: ۴۸ male rats were randomly divided into six ‎groups (n=۸): the sham group that received intraperitoneal normal saline solution (Sham),‎ the HIRI group, the control groups pre-treated with ۵ and ۱۰ mg/kg ZnO-NPs for ۳ ‎consecutive days without surgery (ZnO۵) and (ZnO۱۰), ‎the HIRI group pre-treated with ۵ mg/kg ZnO-NPs‎ for ۳ consecutive days before surgery (HIRI+ZnO۵), ‎and the HIRI group pre-treated with ۱۰ mg/kg ZnO-NPs‎ for ۳ ‎consecutive days before surgery (HIRI+ZnO۱۰)‎. One hour after reperfusion, serum and tissue samples were collected for biochemical, molecular and histopathological evaluation. Results: Administration of ZnO-NPs caused significant improvement in the elevated serum concentrations of ALT, AST, TOS and MDA, improved liver histopathology, and increased TNF-α, IL-۶, and NF-κB levels in liver tissue compared to HIRI group. In addition, administration of ZnO-NPs increased the expression of miR-۱۲۵ in liver tissue compared than in the HIRI group. Conclusion: The administration of ZnO-NPs improved the effect on HIRI by enhancing miR-۱۲۵b expression and suppressing oxidative stress and inflammatory cytokines.

کلیدواژه ها:

Inflammation ، Ischemia-Reperfusion Injury ، Mir-۱۲۵b ، Nanoparticles ، Zinc Oxide.