Minimizing Cytotoxic Effects on Normal Cells: Enhanced Targeted Delivery of Docetaxel via PCL-PEG-PCL Nanocarriers in Breast Cancer Treatment

Nanoparticle-based drug delivery systems are rapidly advancing in cancer therapy, providing reduced toxic side effects and improved efficacy through controlled drug release. This study encapsulated docetaxel (DTX) in a PCL-PEG-PCL matrix using a modified nano-precipitation method, focusing on its cytotoxic effects on breast cancer cell lines. The research aimed to clarify the mechanisms behind the cytotoxic effects and cell death induced by DTX-loaded nano-carriers, utilizing human breast cancer cell lines as an experimental model. The synthesized nano-carriers were characterized through methods such as transmission electron microscopy (TEM), atomic force microscopy (AFM), and dynamic light scattering (DLS). Drug release kinetics and encapsulation efficiency were evaluated with high-performance liquid chromatography (HPLC) and dialysis techniques. The cytotoxicity of DTX-loaded nano-carriers and free DTX was assessed in MCF-۷, MDA-MB-۲۳۱, and fibroblast cell lines over ۴۸ and ۷۲ hours using MTT assays to measure viability and Hoechst ۳۳۳۴۲ staining for apoptosis. Results showed that nano-carriers significantly inhibited cell growth in MCF-۷ and MDA-MB-۲۳۱ cells, especially with formulation P۱ over ۷۲ hours, compared to ۴۸ hours. Apoptosis assays confirmed the effective induction of cell death by nano-carriers. The IC۵۰ values for formulation P۱ were substantially higher after ۷۲ hours. Overall, these findings suggest that the designed nano-carriers promote controlled drug release and enhance apoptotic cell death while reducing the toxic side effects of docetaxel on normal cells, highlighting their potential in cancer therapeutics.

کلیدواژه ها:

Breast cancer ، Cytotoxicity ، Docetaxel ، Nanocarrier ، pcl-peg-pcl