Development of a Core–Shell Nanomagnetic Agarose-Based Solid-Phase Extraction Method Modified with Quercetin for Ultrasensitive Determination of Remdesivir in Serum via HPLC

A magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles wasA magnet-enhanced solid-phase extraction technique utilizing spherical core–shell nanomagnetic agarose particles was devised to extract remdesivir from serum samples. These nanomagnetic agarose particles underwent activation through the epichlorohydrin method and were subsequently modified using quercetin dihydrate as a ligand to facilitate remdesivir extraction. To quantify the target analyte, high-performance liquid chromatography (HPLC) was employed following preconcentration via the developed method. The influence of various analytical variables, including pH, ionic strength, magnet passes, and adsorbent quantity, was systematically examined and optimized using a multivariate central composite design approach. Under optimal conditions, five consecutive analyses demonstrated a remdesivir recovery rate of ۹۹.۴%, with a relative standard deviation of ۳.۶۶%. The method’s detection limit (۳σ) for remdesivir was determined to be ۰.۰۲۷ mg L−۱. This extraction technique was successfully validated for the quantification of remdesivir in serum samples

کلیدواژه ها:

Remdesivir ، Solid-phase extraction ، Nanomagnetic agarose particles ، Quercetin dehydrate