Detection of different species of Brucella in stray dogs using serological and molecular techniques: A strategy for controlling brucellosis in humans and animals

Brucellosis is one of the most common infections in humans and animals. The dog has a main role in maintaining and transmitting this pathogen between humans and livestock. Close contact between dogs, humans, and animals may lead to the transmission of Brucella species, causing shared illnesses in humans and livestock and economic losses from abortion and stillbirth in animals. This research investigated the occurrence of brucellosis in dogs and compared the sensitivity and specificity of the Rose Bengal test with the traditional Wright test for diagnosing brucellosis in dogs. Blood was taken from ۴۶ dog collars without an owner. Rose Bengal, Wright, and ۲-ME tests were performed on serum and PCR was conducted on whole blood. The serum samples were first screened with the Rose Bengal test. All sera were then tested with Wright's or standard tube test and ۲-mercaptoethanol to confirm positive animals. Based on the Rose Bengal test, the prevalence of brucellosis in the studied dogs was ۱۵.۲۱%. The rate of disease prevalence was determined by Wright and ۲-ME methods, ۱۰.۸۶%, and by PCR method, ۱۳.۰۴%. In a titer of ۱:۸۰, the positive Wright test served as the diagnostic threshold. The sensitivity, specificity, and positive and negative predictive values for the Rose Bengal test were ۱۰۰%, ۹۵.۱۲%, ۷۱.۴۲%, and ۱۰۰%, respectively.According to this research, the Rose Bengal test has significant validity in the contamination of dogs with common strains between humans and livestock. It can also be used as a primary screening test with a titer of ۱:۸۰. For a definitive diagnosis, other tests and culture can be used if necessary.

کلیدواژه ها:

Brucellosis Brucella Dogs PCR ، RFLP Serological tests