The Effect of Mg۲+ and Mn۲+ on Over-Production of Interleukin-۲ in Recombinant E.coli

In order to increase the productivity of human interleukin-۲ (IL-۲) as a model protein in the recombinant E.coliBL۲۱ (DE۳), the effect of magnesium acetate and manganese acetate was studied based on full factorial design experiments magnesium acetate and manganese acetate and their interaction exhibited an increasing effect on IL-۲ expression level upto ۲۵.۲۴%, ۱۶.۸۰% and ۲۳.۳۰%, respectively, in comparison to ۱۰.۷۶% for the standard M۹ medium(as usual defined medium in high cell density culture). Magnesium acetate was the most effective salt.Other experiments were performed to investigate the effect of magnesium and acetate ions separately. Results revealed that M۹ medium with ۱.۵ times of magnesium ion concentration resulted in the highest expression level.

کلیدواژه ها:

Recombinant Escherichia coli ، Human interleukin-۲ ، Full factorial design ، Magnesium ، Manganese