Chrysin’s protective effect on the expression of Protamine, Tsga۱۰, Dazl and Akap۴ Genes against diazinon toxin in male rats: An experimental study

Background:Environmental pollution with pesticides is a major health problem worldwide that causes toxicity. Chrysin (C) is a flavonoid that reduces the adverse effects caused by diazinon (D). Objective:To investigate the protective effects of Chrysin on the expression of Protamine, Tsga۱۰(Testis-specific gene antigen ۱۰), Dazl (deleted azoospermia-like gene), and Akap۴(A-Kinase anchoring protein ۴) genes in the testicular tissue of rats receiving D. Materials and methods:In this experimental study, ۴۲ Wistar rats(۷-۸ wk, ۱۸۰-۲۵۰ gr) were selected. This study was conducted in  ۲۰۲۱ at the Pasteur Institute of Iran, North Amol Research Center. Injections were performed ۵ days a week for ۴ wk. Random grouping of rats: ۱. Control:without any injection. ۲. Sham: Injection of ۱۰% Tween ۸۰ solution as D solvent. ۳. D: Injection at a concentration of ۲۰ mg/kg/BW. ۴ and ۵. C with low dose(C۱۰ mg/kg/BW) and high dose(C۲۰ mg/kg/BW). ۶ and ۷: D with low dose of C (D ۲۰ mg/kg/BW/C۱۰ mg/kg/BW) and D with high dose: (D ۲۰ mg/kg/BW/C۲۰ mg/kg/BW). Finally, the testes were examined in terms of histology and gene expression. Results: Histological results showed that D improved spermatogenesis after C intervention by reducing germinal cells, diameter, and lumen area. SPSS results showed that D reduced the expression of Dazl(p < ۰.۰۰۰۱), Tsga۱۰(p = ۰.۰۱), and Protamine(p < ۰.۰۰۰۱) genes, but increased the expression of Akap۴ gene(p < ۰.۰۰۰۱). Conclusion: D has a negative effect on the expression of meiotic genes in rat testicular tissue,while C greatly reduces the adverse effects caused by D.

کلیدواژه ها:

Diazinon ، Chrysin ، Spermatogenesis ، Rat male. Gene Expression. ، دیازینون ، کرایزین ، اسپرماتوژنزیس ، موش صحرایی نر ، بیان ژن.