Enhancing Memory Management in Brain Neurons Post - Spinal Cord Injury with Ellagic Acid -Enriched Fibrous Scaffolds

Impairments in memory and cognition are significant secondary effects of spinal cord injury (SCI) affecting the brain. This study aimed to investigate the impact of implanting a polycaprolactone/functionalized multiwall carbon nanotube (PCL/f -MWCNT) scaffold combined with liposomal ellagic acid (EA@lip) on neurological recovery and brain deficits following SCI. The scaffolds were synthesized by electrospinning of composite fibers followed by EA@lip. Twenty-four female Wistar rats were randomly divided into four groups (n=۶): Ctrl - group (laminectomy without SCI), Ctrl+ group (SCI), PCL/CNT group (implantation of PCL/f -MWCNT scaffold), and PCL/CNT/EA group (implantation of PCL/f -MWCNT/EA@lip scaffold). The injury model involved dorsal hemisection at the T۹ level. Behavioral assessments were conducted to evaluate the neurobehavioral performance of the animals. After four weeks, the mRNA levels of amyloid beta precursor protein (APP), cyclic nucleotide phosphodiesterase (CNP), glutamate ionotropic receptor kainate type subunit ۲ (GRIK۲), and syntaxin-binding protein ۱ (Munc ۱۸-۱) in the brain were analyzed using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results revealed that EA@lip had the desired size, zeta potential, and lipid concentration. Transmission electron microscopy revealed that f-MWCNTs were well-aligned along nanofibers. Ultimate tensile strength, elongation at break, and Young’s modulus of the composite scaffold were obtained at ۳.۳۷ MPA, ۶۱%, and ۱۵ MPa, respectively. Also, UV-Vis spectroscopy demonstrated ۷۰% of ellagic acid release after ۷ days. EA-loaded liposome dramatically enhanced the hydrophilicity of the scaffold. RT-qPCR analysis revealed that CNP and Munc ۱۸-۱ expression levels were elevated, while APP and GRIK۲ expression levels decreased in the groups receiving PCL/CNT with or without EA@lip, relative to the injury group. Furthermore, treatment with PCL/CNT/EA@lip significantly improved behavioral performance compared to the Ctrl+ and PCL/CNT groups. Overall, this study

کلیدواژه ها:

Memory Management ، Brain Neurons ، Spinal Cord Injury ، Ellagic Acid ، Fibrous Scaffolds ، Polycaprolactone ، Multiwall Carbon Nanotube ، Liposomal Ellagic Acid ، Neurological Recovery ، Neurobehavioral Performance