Evaluation of probiotic properties of Enterococcus faecalis andEnterococcus faecium species isolated from rind cheese of Isfahanprovince in 2023

Introduction: Skin cheese is one of the traditional dairy products of different regions ofour country. Enterococci are among the dominant bacteria of cheeses made from raw milk,such as rind cheese, which is even used as starter cultures or probiotics in cheeses. The aimof this study was to isolate and identify Enterococcus faecalis and Enterococcus faeciumbacteria with probiotic properties from rind cheeses of Isfahan province.Materials and methods: For this purpose, after the isolation and diagnosis of enterococciby phenotypic and biochemical methods, the characteristics of resistance in the simulatedconditions of the human digestive system (acid, pepsin, bile and pancreatin), theantagonistic properties of the isolates and the properties of auto-aggregation and co-aggregation with Escherichia coli and Salmonella enterica bacteria was evaluated.Results and discussion: The results showed the presence of enterococci in 23 cheesesamples (76.6%), which were detected by additional and confirmatory biochemical tests of29 isolates of Enterococcus faecium (50%) and 22 isolates of Enterococcus faecalis(37.9%). Among these, in total, 14 samples (27.4%) were able to survive in the presence ofacid and pepsin and were selected for the next steps, and finally 12 isolates also toleratedthe presence of bile and pancreatin well and their antimicrobial and cumulative propertieswere evaluated. was placed The isolates showed significant anti-listerial effects and alsohad a high ability to accumulate with themselves (53.95%) and their cumulative effect withbacteriaSalmonella enterica was obtained more than Escherichia coli. From the results, it can beconcluded that some local isolates of Enterococcus faecalis and Enterococcus faecium inrind cheese have good potential for use as probiotics.

کلیدواژه های Evaluation of probiotic properties of Enterococcus faecalis andEnterococcus faecium species isolated from rind cheese of Isfahanprovince in 2023:

Enterococcus faecalis ، Enterococcus faecium ، antibiotic resistance ، dairyproducts.