Determining the pattern of antibiotic resistance in Enterobacter cloacaeisolates from meat samples in Isfahan province in ۱۴۰۰

Introduction: Usually, contaminated food is one of the main causes of human infections,and poultry meat and sheep meat are important sources. Enterobacter cloacae strains,having different virulence factors and multiple antibiotic resistances, are mainly consideredas an opportunistic pathogen.Materials and methods: In this research, isolation of Enterobacter cloacae ۳۸۴ fromchicken and sheep meat was done randomly in ۱۴۰۰ in Isfahan province by microbial andmolecular methods. Antibiotic resistance pattern was determined by disc diffusion methodand microtitre plate method was used to investigate biofilm production. The ability toproduce broad-spectrum β-lactamase enzymes was investigated through phenotypic andgenotypic methods.Results and discussion: Out of ۳۸۴ examined samples, Enterobacter cloacae wasidentified as Enterobacter cloacae in ۲۵ samples (۶.۵۱%), which were also confirmed inthe presence of hsp۶۰ gene in the molecular analysis. Among these, ۱۸ samples wererelated to chicken meat (۷۲%) and ۷ samples (۲۸%) were related to sheep meat. Among theisolates, the highest antibiotic resistance to cotrimoxazole and cefotaxime was reported in۲۰ isolates and the lowest resistance to nitrofuranthein in ۱۵ isolates (۲۳.۸%). In microtitermethod, ۱۵ isolates (۶۰%) showed strong biofilm reaction, ۱۰ isolates (۴۰%) showedmoderate biofilm reaction. The present study indicates that ESBL producing strains ofEnterobacter cloacae have a relatively high prevalence. The increase in the amount of thesestrains is often caused by the irrational prescription of antibiotics, which requires the use ofnew antimicrobial agents to solve this problem.

کلیدواژه ها:

Enterobacter cloacae ، biofilm ، antibiotic resistance ، meat products.