The effects of p-benzoquinone and hydroquinone on the RUNX۲ gene expression and osteoblastic differentiation of human marrow derived mesenchymal stem cells

Objective: Evaluating the effects of p-benzoquinone and hydroquinone on the RUNX۲ expression and osteoblastic differentiation of human marrow derived mesenchymal stem cells (MSCs). Materials and Methods: Bone marrow MSCs obtained by cultivating marrow mononuclear cells, were exposed to ۱۰μM of either p-benzoquinone or hydroquinone. Following chemical treatment, RUNX۲ gene expression was assessed by Real-time RT PCR ۱, ۶, ۲۴ and ۴۸ hours later and osteogenic differentiation was analyzed using alizarin red and alkaline phosphatase staining methods on days ۷ and ۱۴ after ostegenic induction. Results: RUNX۲ expression was significantly elevated (up to approximately ۸ times) due to chemical exposure but the applied chemicals exert no considerable effect on MSCs osteogenic differentiation. Conclusion: According to the literature, despite the necessity of RUNX۲ overexpression on the induction of osteogenic differentiation, but it is not sufficient for osteogenesis to occure so increase in RUNX۲ expression observed in our study is not the indicator of the induced osteogenic differentiation. Instead, this elevated expression could be the sign of increased activity of the canonical Wnt signaling pathway thereby its involvement in the development of AML due to exposure to benzene and its metabolites. Moreover, this augmented expression of RUNX۲ in MSCs can indicate the RUNX۲ overexpression in myeloid progenitors as an expected similar effect of exposure to benzene and its metabolites to contribute in myeloid malignancies developed due to benzene exposure.

کلیدواژه ها:

Human Marrow Derived Mesenchymal Stem Cells (MSC) ، Runx۲ Gene ، Osteogenic Differentiation ، Hydroquinone (HQ) ، &rho ، -Benzoquinone (BQ) ، تمایز استئوبلاستی ، سلول های بنیادی مزانشیمی (MSC) انسانی ، ژن RNUX۲ ، پارابنزوکواینون ، هیدروکواینون