Protective effects of resveratrol on the expression of catalase, glutathione peroxidase, and superoxide dismutase genes in the ovary and their activity in the serum of rats exposed to lead acetate: An experimental study

Background: Lead (Pb) could be toxic to the female reproductive system, and resveratrol (Res) may overcome this toxicity. Objective: To investigate the Res impact on the catalase (Cat), glutathione peroxidase (Gpx), and superoxide dismutase (Sod) gene expression in the ovary and on the Cat and Gpx enzyme activity in the serum of rats exposed to lead acetate. Materials and Methods: In this experimental study, ۳۳ female Wistar rats (۸-۱۰ wk, ۱۸۰-۲۰۰ gr) were divided into ۶ groups: a control group (normal saline), a Res group (۴۰ mg/kg), and a Pb group (lead acetate ۳۰ mg/kg). ۳ additional groups received lead acetate (۳۰ mg/kg) with Res at ۲۰, ۴۰, and ۸۰ mg/kg for ۲۱ days. Gene expression of Cat, Gpx, and Sod was measured via qPCR, and serum Cat and Gpx activity was assessed using standard methods. Bioinformatics tools were used to evaluate Res effects on gene and protein function. Results: Lead acetate significantly downregulates Cat, Gpx, and Sod gene expression, but Res significantly upregulates gene expression, especially at doses of ۴۰ mg/kg for Cat, ۲۰ mg/kg and ۴۰ mg/kg for Gpx, and ۸۰ mg/kg for Sod. Cat and Gpx enzyme activity increased and decreased in the lead acetate group, respectively. However, Res in all doses decreased only the Cat enzyme activity. Bioinformatics analysis indicates that Res can interact with the promoter regions and cavities of all ۳ enzymes. Conclusion: Pb can dysregulate the expression and activity of the studied enzymes. However, the impact of Res is influenced by the dose, with ۴۰ mg/kg frequently being the most effective.

کلیدواژه ها:

Resveratrol ، Lead acetate ، Oxidative stress ، Ovary ، In silico ، Rat. ، رسوراترول ، استات سرب ، استرس اکسیداتیو ، تخمدان ، درون رایانه ای ، موش صحرایی.