β-cyclocitral Extracted from Galium mite (medicinal herb) and the Evaluation of its Effect in MCF-۷ Cell Lines

Carotenoids play important role as antioxidant and induction of gene expression changes lead to plants acclimation to environmental stresses. Βetacyclocitral (BCC) derived from Galium mite Boiss. & Hohen. in the process of β-carotene oxidation has recently proved that has a new function in breast cancer treatment. In this project we used drug loaded delivery released system (DDRS) strategy, coupled with super paramagnetite nanoparticles (SNPs) based on graphene quantum dots (GQDs) synthesis to deliver drugs to tumor sites. The validation of synthesized SNPs was implemented with several techniques such as Fourier-transform infrared (FT-IR) spectroscopy, X-ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscope (TEM). MCF-۷ cell lines were treated with GQD, β-cyclocitral and GQD@β-cyclocitral (each with concentration of ۳.۹۳, ۷.۸۷, ۱۵.۷۵, ۳۱.۲۵, ۶۲.۵, ۱۲۵, ۲۵۰, ۵۰۰, and ۱۰۰۰ µg/ml) in MTT assay in ۲۴ and ۴۸ hours respectively. IC۵۰% was calculated in ۲۴ h.  Results indicated that β-cyclocitral had prominent effects in breast cancer cell lines controlling.

کلیدواژه ها:

β-cyclocitral ، Breast cancer ، Nano-encapsuulate ، Graphene quantum dot (GQD) ، drug delivery