A Graphene Reinforced Fabric Phase Sorptive Extraction Method for Determination of Ochratoxin A in Food Samples

In the present study, a novel sorbent-based microextraction method was developed for the quick extraction of ochratoxin A from food samples. The extraction was performed based on graphene-reinforced fabric phase sorptive extraction (FPSE) followed by HPLC-FLD analysis. Chromatographic separations were performed on a C۱۸ column with H۲O: ACN: acetic acid (۴۹:۴۹:۲, v/v/v) as mobile phase at a flow rate of ۱.۰ mL/min and with fluorescence detection (λex= ۳۳۳ nm and λem= ۴۴۷ nm). The effect of graphene in the sol solution, extraction time, ionic strength, solvent extraction volume, desorption time, and desorption solvent type and volume were evaluated to obtain the maximum extraction efficiency. The optimum condition was obtained at pH=۷, ۴۰ mg of graphene per ۱۰ mL of sol solution, ۴۰ min for extraction time and ۲۰ min for desorption time, the best solvent for desorption was ۱ mL of ACN: HOAC (۹۵:۵ V/V) and extraction volume was ۱۰ mL. The method showed a linear range of ۲.۰-۱۷.۵ ng/mL with a correlation coefficient greater than ۰.۹۷. The limit of detection and limit of quantification were found ۰.۴۹ ng/g and ۱.۴۹ ng/g, respectively with absolute good recoveries (۶۷.۷-۱۰۴.۰%) and low relative standard deviations. In addition, ochratoxin A was quantified with the developed method in wheat and chickpea samples and the results indicate the effectiveness of the presented method.

کلیدواژه ها:

Mycotoxin ، Chickpea ، Pre-concentration ، Sportive extraction