Evaluation of the sperm parameters, oxidative stress, and histopathological effects of vitamin B۱۲ in preventing Helicobacter pylori-induced testicular toxicity: An experimental study

Background: Helicobacter pylori (H. pylori) causes sterility by affecting the reproductive system. Vitamin B۱۲ improves sperm quantity and function. Objective: Vitamin B۱۲ protection against H. pylori adverse effects was investigated. Materials and Methods: ۴۰ C۵۷ male mice (۶ wk) were randomly assigned to ۴ equal groups (n = ۱۰) including, group ۱ (control without any intervention), group ۲ (H), ۳ (HP), and ۴ (HB) received ۱×۱۰۹ colony forming unit (CFU) of H. pylori, ۱×۱۰۹ CFU of H. pylori+phosphate buffered saline, ۱×۱۰۹ CFU of H. pylori+۵۰ μg/kg vitamin B۱۲ intraperitoneally, respectively. In the induction groups, the H. pylori was orogasterically injected ۳ times with ۱ cc phosphate buffered saline throughout the day. Then testicular metrics, sperm motility, viability, quantity, and shape, plasma levels of malondialdehyde (MDA), superoxide dismutase, glutathione peroxidas, and total antioxidant capacity were measured. Also, testicular-tissue changes were examined using Johnson scores, tubular differentiation index, and spermatogenesis index. Vitamin B۱۲, homocysteine, and testosterone serum levels were examined. Results: The results showed a significantly lower Johnson score, tubular differentiation index, and spermatogenesis index, and serum level of testosterone and homocysteine as well as a higher MDA level in the H and HP groups than the HB group (p < ۰.۰۵). In contrast, the highest superoxide dismutase and glutathione peroxidase enzymes activity and total antioxidant capacity as well as the lowest serum level of MDA were found in the HB group compared to other groups (p < ۰.۰۵). Conclusion: Vitamin B۱۲ increased antioxidant enzyme activity, enhanced sperm parameters, and decreased injury to testicular tissue. It can be used as a potent antioxidant in reducing testicular damage induced by H. pylori.

کلیدواژه ها:

Helicobacter pylori ، Reproductive medicine ، Oxidative stress. ، : هلیکوباکتر پیلوری ، پزشکی تولید مثل ، استرس اکسیداتیو.