Ca۱۹Zn۲(PO۴)۱۴ Nanoparticles: Synthesis, characterization and its effect on thecolonization of Streptococcus mutans on tooth surface

The main cause of tooth decay is the biofilmformation of Streptococcus mutans. This studyaimed to investigate the effect of the zinc oxidenanoparticles (ZnO NPs), hydroxyapatitenanoparticles (Ca۱۰(PO۴)۶(OH)۲) (HAP NPs), zincoxide/hydroxyapatite nanocomposites (ZnO/HAPNCs), and Zn substituted hydroxyapatitenanoparticles (Ca۱۹Zn۲(PO۴)۱۴ NPs) on the growthand biofilm formation, bacterial adherence, and theexpression of ftf and gtfC genes in Streptococcusmutans.Materials and MethodsThe nanostructures were prepared via simple andfast co-precipitation route. Twelve isolates ofStreptococcus mutans collected from children withdental caries referred to the dental clinic of KashanUniversity of Medical Sciences. All S. mutansisolates were susceptible to Ampicillin.Results and DiscussionThe mean MIC for ZnO NPs, HAP NPs,Ca۱۹Zn۲(PO۴)۱۴, and ZnO/HAP NCs were ۱۱۸, ۲۶۰,۷۰.۶, and ۹۹۴ mg/mL, respectively. All preparednanostructures significantly reduced biofilmformation at MIC and sub-MIC concentrations (p <۰.۰۱). In biofilm and cell culture treated withnanoparticles, the expression of ftf and gtfC genesdecreased. Results were shown that IC۵۰ for theCa۱۹Zn۲(PO۴)۱۴ was ۸.۵, and for non-toxicconcentration, was ۰.۰۶۵ mg/mL. The attachmentrate to the denture surface and HGF۱ cell linetreated with the Ca۱۹Zn۲(PO۴)۱۴ NPs hasdecreased.ConclusionsThe results showed that the Ca۱۹Zn۲(PO۴)۱۴ NPshas a better effect than the ZnO/HAP NCs.It can therefore be used as a coating on dentalsurfaces. Investigation of Ca۱۹Zn۲(PO۴)۱۴ NPsform for covering dental teeth surfacerecommended in future study.

کلیدواژه ها:

Hydroxyapatite ، Nanocomposites ، Biofilm ، MIC ، Dental