Zinc Oxide Nanoparticles Ameliorate Histological Alterations Through Apoptotic Gene Regulation in Rat Model of Liver Ischemia-Reperfusion Injury

Background: Organ ischemia-reperfusion (IR) is a common clinical condition associated with various situations such as trauma surgery, organ transplantation, and myocardial ischemia. Current therapeutic methods for IR injury have limitations, and nanotechnology, particularly zinc oxide nanoparticles (ZnO NPs), offers new approaches for disease diagnosis and treatment. In this study, we investigated the protective and anti-apoptotic effects of ZnO NPs in liver ischemia-reperfusion (IR) injury in rats. Methods: Forty-eight male rats were divided into six groups: sham, ZnO۵, ZnO۱۰, ischemia-reperfusion (IR), IR+ZnO۵, and IR+ZnO۱۰. The protective effect of ZnO NPs was evaluated by liver enzymes (AST, ALT, Bilirubin, ALP), biochemical (TAC, TNF-α, and MDA), molecular examinations (Bcl۲, BAX), and histopathological evaluations (H&E, TUNEL). Results: Pre-treatment with ZnO۵ and ZnO۱۰ improved hepatic function in IR liver injury, attenuated the levels of oxidants (P = ۰.۰۳) and inflammatory mediators, and reduced apoptosis (P = ۰). ZnO۱۰ was found to have a greater effect on ischemic reperfusion injury than ZnO۵ did. Histopathological examination also showed a dose-dependent decrease in alterations in the IR+ZnO۵ and IR+ZnO۱۰ groups. Conclusion: Administration of ZnO۵ and ZnO۱۰ improved liver function after IR. The findings of this study suggest that ZnO NPs have a protective effect against oxidative stress and apoptosis in liver ischemia-reperfusion injury in rats. These results may have important implications for developing advanced methods in ischemia-reperfusion treatment. Keywords: Anti Apoptotic Protein, Caspase ۳, Metal Nanoparticle, Zinc Compounds. Background: Organ ischemia-reperfusion (IR) is a common clinical condition associated with various situations such as trauma surgery, organ transplantation, and myocardial ischemia. Current therapeutic methods for IR injury have limitations, and nanotechnology, particularly zinc oxide nanoparticles (ZnO NPs), offers new approaches for disease diagnosis and treatment. In this study, we investigated the protective and anti-apoptotic effects of ZnO NPs in liver ischemia-reperfusion (IR) injury in rats. Methods: Forty-eight male rats were divided into six groups: sham, ZnO۵, ZnO۱۰, ischemia-reperfusion (IR), IR+ZnO۵, and IR+ZnO۱۰. The protective effect of ZnO NPs was evaluated by liver enzymes (AST, ALT, Bilirubin, ALP), biochemical (TAC, TNF-α, and MDA), molecular examinations (Bcl۲, BAX), and histopathological evaluations (H&E, TUNEL). Results: Pre-treatment with ZnO۵ and ZnO۱۰ improved hepatic function in IR liver injury, attenuated the levels of oxidants (P = ۰.۰۳) and inflammatory mediators, and reduced apoptosis (P = ۰). ZnO۱۰ was found to have a greater effect on ischemic reperfusion injury than ZnO۵ did. Histopathological examination also showed a dose-dependent decrease in alterations in the IR+ZnO۵ and IR+ZnO۱۰ groups. Conclusion: Administration of ZnO۵ and ZnO۱۰ improved liver function after IR. The findings of this study suggest that ZnO NPs have a protective effect against oxidative stress and apoptosis in liver ischemia-reperfusion injury in rats. These results may have important implications for developing advanced methods in ischemia-reperfusion treatment.

کلیدواژه ها:

Anti Apoptotic Protein ، Caspase ۳ ، Metal Nanoparticle ، Zinc Compounds.