Preservation of Buck Semen Quality During Chilling Storage Using Coenzyme Q۱۰

Background: Storing spermatozoa in a cold environment decreases spermatozoa metabolism while maintaining sperm fertility. Because of their unique physiological characteristics, sperm cells’ reproductive ability in small ruminants is reduced by cooling.Objectives: This study aimed to assess the impact of supplementing chilling medium with coenzyme Q۱۰ (CoQ۱۰) on the quality parameters of buck’s semen after chilling at ۴°C.Methods: Semen was collected, diluted, and divided into five groups with different CoQ۱۰ supplementation concentrations (۰, ۱, ۲, ۵, and ۱۰ µM CoQ۱۰). Collected semen were stored at ۴°C for ۵۰ h. The motility, mitochondrial activity, membrane integrity, viability, and lipid peroxidation were measured after ۰, ۲۵, and ۵۰ h of chilling.Results: According to the findings, different concentrations of CoQ۱۰ had no impact (P>۰.۰۵) on semen at time ۰ of storage. After ۲۵ and ۵۰ h of storage, supplementing the chilling medium with ۵ µM CoQ۱۰ increased (P≤۰.۰۵) progressive motility (PM), total motility (TM), viability, mitochondrial activity, and membrane integrity compared to other groups. Furthermore, ۵ µM CoQ۱۰ produced less lipid peroxidation (P≤۰.۰۵) after ۲۵ and ۵۰ h than the other groups.Conclusion: Adding ۵ µM CoQ۱۰ to the buck’s semen cooling medium is an effective procedure for protecting the quality of buck spermatozoa during cooling storage.

کلیدواژه ها:

Cooling ، CoQ۱۰ ، Goat ، Quality ، Semen