Investigating the Effectiveness of Spirocyclopropane-Oxindole Derivatives on Clinical Isolates of Candida albicans

Objectives: Given the spread of azole resistance in Candida albicans (C. albicans), searching for new potent compounds, such as spirocyclopropane-oxindole derivatives is important. This study evaluates the antifungal susceptibility of spirocyclopropane-oxindole derivatives on clinical isolates of C. albicans.  Methods: Antifungal susceptibility of ۵۰ clinical isolates of C. albicans to spirocyclopropane-oxindole derivatives (۴a, ۴b, and ۴c), nystatin, and fluconazole were evaluated according to Clinical Laboratory Standards Institute (M۲۷-S۴) guidelines. The medicinal dilution range of the compounds, fluconazole, and nystatin was ۰.۲۵۶ to ۱۲۸, ۰.۱۲۸ to ۶۴, and ۰.۰۳۲ to ۱۶ μg/mL, respectively. The minimum inhibitory concentration (MIC) was defined as the concentration that caused at least ۵۰% growth inhibition compared to the positive control. Statistical analysis was performed using the SPSS software, version ۲۰. The significance level was set at P≤۰.۰۵. Results: There was a significant difference between the MIC values of spirocyclopropane-oxindole derivatives (۴a, ۴b, and ۴c), nystatin, and fluconazole against C. albicans. The comparison of the MICs of the spirocyclopropane-oxindole derivatives (۴a, ۴b, and ۴c) against C. albicans showed that derivative ۴a had a lower MIC۵۰ (۸ μg/mL), MIC۹۰ (۱۶ μg/mL), and Geometric (G) Mean (۱۰.۱۲۶) than derivatives ۴b (MIC۵۰=۶۴, MIC۹۰=۱۲۸, G Mean=۷۶.۶۳۸), and ۴c (MIC۵۰=۶۴, MIC۹۰=۱۲۸, G Mean=۶۰.۵۴۷). Discussion: Antifungal effects of spirocyclopropane-oxindole derivatives (۴a, ۴b, and ۴c) on C. albicans isolates were significantly less than nystatin and fluconazole. Therefore, with structural changes, the antifungal effects of these compounds will increase.

کلیدواژه ها:

Spiro-cyclopropane-oxindoles derivatives ، Fluconazole ، Nystatin ، Candida albicans