Apoptotic potential of biogenically synthesized selenium nanoparticles with Lactobacillus casei supernatant against breast cancer cells

BACKGROUND AND OBJECTIVESNowadays, cancer is the most common life-threatening disease which is spreading due to lifestyle. The use of nanoparticles in biomedical research has been increasingly developed in recent years. The aim of this study was to investigate the anticancer effects of biogenic synthesized selenium nanoparticles (SeNPs) using cell-free supernatant of Lactobacillus casei on MCF-۷ cancer cell line.MATERIALS AND METHODSThe prepared SeNPs were characterized by XRD, Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and energy dispersive spectroscopy (EDX), and Zeta potential.RESULTS AND DISCUSSION The SEM images demonstrated the spherical morphology of the synthesized nanoparticles. Treatment of breast cancer cells with different concentrations of nanoparticles showed that the nanoparticles had the ability to inhibit MCF-۷ proliferation, and flow cytometry showed that SeNPs could induce apoptosis in MCF-۷ cells. Gene expression analysis showed that after treatment of MCF-۷ cells with SeNPs, the expression levels of CASP۳, CASP۹ and BAX genes were increased. SeNPs could also prevent migration and invasion of MCF-۷ cancer cells.CONCLUSION These finding indicated that SeNPs induced G۱ phase arrest and apoptosis in MCF-۷ cells. The process used in this study to develop the nanoparticles is novel and should have useful anticancer applications in the future, mainly because of proper specific targeting to cancer cells. SeNPs may serve as a potential therapeutic agent for breast cancer

کلیدواژه ها:

Apoptosis ، Breast Cancer ، Lactobacillus casei ، Selenium Nanoparticles ، Cell cycle arrest