Identification and comprehensive analyses of the CBL gene families in sweet orange (Citrus sinensis)

Calcineurin B-like (CBL) proteins, as calcium sensors, serve key roles in plant responses to varied abiotic stressors and in growth and development through interaction with CBL-interacting protein kinases (CIPKs). However, information on the roles and development of CBLs in sweet orange plants is limited. We surveyed the whole Citrus sinensis genome and found eight CBL genes. Domains features, position and distribution and conserved motif revealed that EF-hands domain was fairly conserved across the eight CsCBLs. CsCBL proteins are classed as acidic CBL, and five myristoylation sites and six palmitoylation sites were predicted. Eight CsCBLs were distributed on four chromosomes (Chr۰۱, Chr۰۲, Chr۰۴ and Chr۰۵) and one contig (chrNW-۰۰۶۲۵۷۱۰۴.۱). Two CsCBL genes (CsCBL۴ and CsCBL۵) were identified in Cschr۰۵ that were tightly linked, and likely to have been created through tandem duplications. Phylogeny tree of ۳۷ CBL protein from different plant species including Arabidopsis thaliana, Oryza sativa, sesamum indicum and C. sinensis showed that these CBLs are closely related. The results of this study highlight the functional properties of the CsCBL gene family and give crucial data for future research into their functional activities.

کلیدواژه ها:

Calcineurin B-like ، Calcium sensor ، CBL ، Citrus sinensis ، Sweet orange