Effect of experimental infectious bursal disease virus on clinical signs and pathogenesis of avian influenza virus H۹N۲ in turkey by real time PCR

Infectious bursal disease virus (IBDV) in turkeys may result in immunosuppression, and inability of turkeys to resist nonpathogenic or less pathogenic organisms. A total number of ۱۲۰ day-old commercial male turkeys were purchased and blood samples were collected from ۲۰ day-old turkeys, remaining ۱۰۰ were divided into four equal groups and kept in separated rooms. Groups ۱ and ۲ were infected with ۱۰۴ CID۵۰ of IBDV via intra-bursal route on day ۱; Groups ۱ and ۳ were each infected with ۱۰۶ EID۵۰of AIV (H۹N۲) via the oculo-nasal routes on day ۳۰. All groups were vaccinated against Newcastle disease vaccine (NDV). Detection of avian influenza virus H۹N۲ in trachea and cloaca swabs and in the tissues, was confirmed by Real-time polymerase chain reaction. Anti- NDV–AIV and anti-IBD titers were measured using HI and ELISA tests, respectively. The present study showed that infectious bursal disease changed the pathogenesis of (AIV) H۹N۲ by affecting AI virus replication and resulted in an increase shedding due to prolonged duration of sever clinical signs. The extent of shedding and virus replication need further study.

کلیدواژه ها:

Avian influenza ، Infectious bursal disease virus ، Real time PCR ، Turkey