Metabolic and Enzymatic Responses of Calendula officinalis L. to Foliar Application of Spermidine, Citric Acid and Proline under Drought Stress in a Post Harvest Condition

The first experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (۰, ۵۰ and ۱۰۰ mg L-۱) on some metabolites and enzymatic activities of Calendula officinalis L. under drought stress (No stress: Control, ۲۵, ۵۰, and ۷۵% field capacity). The second experiment was performed to study the effect of foliar application of spermidine, citric acid and proline (۰, ۵۰ and ۱۰۰ mg L-۱) on the post-harvest shelf life of flowers (beginning of the experiment, ۵, and ۱۰ days post-harvest). The experiments were factorial in a completely randomized design with ۳ replications. All treatments had a significant effect on the measured variables. In the first experiment, FC ۲۵% reduced all traits, FC ۷۵% increased carotenoid, carbohydrate, phenol, flavonoid, protein, Peroxidase (POD) and Superoxide Dismutase (SOD) activities. The highest vitamin C was observed in the control FC. Also, foliar application of ۱۰۰ mg L-۱ proline, increased carotenoid, carbohydrate and phenol, ۵۰ mg L-۱ proline increased protein content. Also, ۱۰۰ mg L-۱ of spermidine increased flavonoid and ۱۰۰ mg L-۱ of citric acid increased vitamin C, SOD and POD activities. In the second experiment, all evaluated traits were reduced ten days after harvest, the highest post-harvest life was for ۱۰۰ mg L-۱ of citric acid (۹.۷ days) and the lowest was for the control (۵.۳ days). The study results showed that application of ۱۰۰ mg L-۱ spermidine, citric acid and proline with FC ۷۵% improved biochemical properties, nutritional traits, and post-harvest life of Pot marigold.

کلیدواژه ها:

Medicinal and ornamental plants ، Pot marigold ، Shelf life.