Designing a specific nanoprobe for rapid biosensing of Alzheimer’s disease

Nano-biosensors have attracted many attentions since they are sensitive and precise for detection small numberof biomarkers in any environment. Alzheimer’s disease is known as a neurodegenerative disease in which thesymptoms appear in the late stages with cognitive impairment problems. Early diagnosis of Alzheimer’s diseasewould be the first promising step toward the treatment of the disease. This study employs the colorimetric featureof gold nanoparticles for detection of a specific biomarker for diagnosis of Alzheimer’s disease in early stages.The characteristics of the developed nano-biosensor are determining using DLS, zeta potential and FT-IR test.The DLS results show size of ۴۰ nm for the synthesized nanoparticles and ۱۵۰ nm for nanoprobe implying accurateconjugation of the probe with nanoparticles. The zeta potential results showed an increased negative chargefor the functionalized nanoparticles. Investigation of nanoprobes and nanoparticles revealed that the probe conjugatedto nanoparticles properly.

کلیدواژه ها:

Alzheimer’s disease ، nucleic acid biomarker ، Au nanoparticles ، early diagnosis ، nano-biosensor