Targeted Glutathione-Sensitive Nanoparticles for Doxorubicin Delivery to Breast CancerCells

Chitosan (Cs), a biocompatible and biodegradable polymer, has been widely used as a potential nanocarrier inrecent years. Although, the positive charge of the Cs has limited its utilization as an effective nanoparticle (NPs)for the positive charge drugs. So, loading the DOX, a positive charge anticancer drug, into chitosan-based NPsis challenging. Herein, we offer a Cs derivation polymer by covalent linkage to L-Cysteine (L-Cys) for reducingthe enhancing the entrapment efficiency (EE%) of DOX. Accordingly, the targeted DOX-loaded NPs basedon Cs-Cys copolymer with redox-responsive properties were synthesized. At first, the Cs-CysNPs-DOX weresynthesized by ion-gelation method, then they were targeted by Hyalouronic acid (HA). Ultimately, they werecharacterized in terms of size, zeta potential, EE%, and morphology. Also, in vitro drug release was analyzed.Overall, the suggested NPs demonstrated a high potential for targeted therapy and effective applications in biomedicalresearch.

کلیدواژه ها:

Breast cancer ، Chitosan ، Doxorubicin ، Hyaluronic acid ، Glutathione