Quantification of Leghemoglobin Extracted from Roots of Five Different Alfalfa (Medicago sativa) Cultivars: An Experimental Study
محل انتشار: فصلنامه تغذیه و امنیت غذایی، دوره: 8، شماره: 3
سال انتشار: 1402
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 74
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شناسه ملی سند علمی:
JR_JNFS-8-3_009
تاریخ نمایه سازی: 24 مرداد 1402
چکیده مقاله:
Background: Leghemoglobin is an oxygen transporter and a hemoprotein found in nodule roots of nitrogen fixator plants from Leguminosae family. Leghemoglobin is produced in response to presence of bacteria belonging to Rhizobia family. Structurally, leghemoglobin is similar to hemoglobin and has a kind of heme iron structure. Study of leghemoglobin as a heme iron containing agent can open up a new way for treatment and natural prevention of iron deficiency anemia and substitution with mineral iron salts. Methods: Five different cultivars of alfalfa plants including Esfahani, Bammi, Yazdy, Nik shahri, and Hamedani were cultivated in gardening farm of the faculty of agriculture in Shahid Chamran University in a linear form. Cultivation was done with biological fertilizer containing Sinorhizobium meliloti for test groups and without fertilizer for sham groups. After ۶۰ days, the samples were harvested, and the amount of leghemoglobin produced in nodules was quantified using LaRue and Child fluorometric method. Results: The results from five cultivars of alfalfa planted with biological fertilizer showed a significant increase in viscosity of leghemoglobin in nodules of the test group in comparison with sham group (P<۰.۰۰۰۱). Moreover, the high viscosity of leghemoglobin in nodules of Bammi cultivar indicated the capacity of this plant to produce leghemoglobin in Ahvaz. Conclusion: According to the findings of this study, Bammi cultivar of alfalfa is a good option for future studies and even production of high amounts of leghemoglobin in Khuzestan Province.
کلیدواژه ها:
نویسندگان
Amir Siahpoosh
Medicinal Plants Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahwaz, Iran
Mohammad Reza Siahpoosh
Department of Agronomy and Plant Breeding, College of Agriculture, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Soroush Alaei
Department of Pharmacognosy, Faculty of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
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