Detection and Evaluate the Frequency of Cytomegalovirus and Epstein-Barr Virus in Bladder Tumor Samples in Hamedan Province, ۱۳۹۲

Background and Aim: Some unknown reasons as the cytomegalovirus (CMV) and Epstein-Barr virus (EBV) in the incidence of bladder cancer has been much discussed in recent years. The purpose of this study was to identify and Evaluate the frequency CMV and EBV in bladder tumors. Materials and Methods: Fourty samlps of TCC bladder carcinoma collected from the pathology department of the dr Gharazi hospital in hamedan during summer ۲۰۱۲. samples deparaffinized and DNA extracted  by tissue kit (Qiagen). The human beta-globin gene amplified to ensure the accuracy and integrity of the extracted DNA. To perform the PCR, primers amplified BRLF۱ gene for EPV and UL۱۲۳ for CMV.  Therefore amplified fragments were observed on ۱.۵% agarose gel. Finaly  the chi-square test were used to assess the relationship between viruses and their relationship to the incidence of bladder cancer. Results: From ۴۰ carcinoma samples studied ۴ (۱۰%) were infected with the CMV virus and ۲۹ samples (۷۲.۵%) were infected with EBV. Four samples were simultaneously infected with both viruses. The relationship between age, tumor stage, CMV and EBV virus wasn’t observed (P <۰.۰۵).. Conclusions: CMV and EBV can be detected by optimized PCR protocol from bladder cancer. No significant association was observed. The high prevalence of EBV develops this theory that EBV was a factor in bladder cancer.

کلیدواژه ها:

Bladder carcinoma ، PCR ، CMV ، EBV ، کارسینوم مثانه ، PCR ، CMV ، EBV