Novel Combination of Periodontal Ligament Stem Cells (PDLSCs)-Conditioned Medium and Taurine Increase Apoptosis in U۸۷Glioma Tumor Cells: Possible Relevance to Glioblastoma Treatment

Background & Objective: Glioblastoma Multiforme is a very aggressive primary brain cancer that has a median overall survival even if the treatment period is less than ۱ year. Despite progress in glioblastoma diagnosis and treatment, the prognosis of patients with glioblastoma still remains poor. Recently, it has been detected that stem cell therapies are an effective method for brain tumor cell targeting. Also, the anti-inflammatory and anti-apoptotic activity of taurine against cancerous cells has been found. Materials & Methods: In this study, we employed taurine as an anti-cancer drug and PDLSCs as a potential agent for improving anti-cancer drug efficiency. Then, we investigated their effect on the glioma tumor cells in in-vitro ۲D cell culture and in vivo. Results: Taurine had the best apoptotic activity on the C۶ glioblastoma cells at the concentration of ۸۰ μM after ۷۲h post-treatment. The obtained results showed that a combination of taurine/PDLSCs induced the expression of caspase-۳, caspase-۸, and IL-۱۷Ra and down-regulated the expression of Bcl-۲ and IL-۱۷Ra. Uses of taurine and PDLSCs suppress the migration of the cancerous C۶ cells after ۴۸h and show good potential in the suppression of GBM metastasis. Conclusion: The taurine at concentration of ۸۰µM has a strong potential in decreasing the viability of cancerous C۶ Glioblastoma cells. Therefore, taurine and PDLSCs combination, due its therapeutic efficacy, has a considerable potential to be a successful method to glioblastoma brain cancer treatment.

کلیدواژه ها:

Glioblastoma Multiform ، Taurine ، Periodontal Ligament Stem Cells ، Apoptosis