Introduction and aims: Nowadays, the biosynthesis of metallic nanoparticles is on a sharp rise because of its potential in eliminating antibiotic-resistant bacteria. So that, this study reports an eco-friendly and cost-effective methodology for synthesizing biogenic silver nanoparticles (AgNPs) using alcoholic extract of
Peucedanum officinale (PO@AgNP).Material & Methods: In this research, we used
Peucedanum officinale for the green synthesis of silver nanoparticles. In this study, various parameters such as concentration, time and temperature were investigated to achieve optimal conditions to synthesis PO@AgNP. All stages of reaction were monitored by UV-Vis spectroscopy. The PO@AgNPs were characterized in terms of structural properties and morphology with X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR) and transmission electron microscopy (TEM) techniques. The broth microdilution method was performed to determine the antibacterial activity of PO@AgNPs. The antioxidant activities of PO@AgNPs were examined as a percentage of DPPH inhibition.Results: Examination of the XRD data confirmed the synthesis of crystalline particles. The FT-IR spectra showed that phenolic compounds in
Peucedanum officinale extract act as a reducing agent of silver ions and formation PO@AgNPs. TEM images showed that PO@AgNPs had a spherical morphology with a size of about ۳۰-۴۰ nm. PO@AgNPs shown strong antibacterial activity against Klebsiella pneumoniae ,Enterococcus faecalis and Streptococcus mitis with minimum inhibitory concentrations (MIC) values were ۳۱.۲, ۶۲.۵ and ۶۲.۵ µg/ml, respectively. DPPH test have shown that with increasing the concentration of PO@AgNPs (۶۲.۵ to ۵۰۰ μg/ml), the percentage of radical inhibition of DPPH increased (۲۵% to ۵۵%).Conclusion: All investigations showed that PO@AgNPs have high antibacterial activity against gram-positive and negative bacteria and have strong antioxidant properties and introduces it as a very efficient, eco-friendly and cost-effective nanoparticle.