Detection of Genes Encoding Metallo-beta-lactamases in Carbapenem Resistant Acinetobacter baumannii

Background: Drug resistant Acinetobacter baumannii have emerged as a major problem in many hospitals and intensive care units. The aim of this study was to determine the antibiotic resistance pattern and the prevalence of metallo-beta-lactamase genes among nosocomial A. baumannii isolates from Qom/ Iran.Methods: For this study, a total of ۱۰۸ A. baumannii isolates were collected from hospitalized patients in four teaching hospitals of Qom/ Iran. Antibiotic susceptibility profile of isolates was tested by Kirby-Bauer disc diffusion method and distribution of MBL genes among carbapenem-resistant isolates was determined by polymerase chain reaction (PCR) method.Results: According to the results, ۹۷ (۸۹.۸۱%) isolates of ۱۰۸ A. baumannii isolates were resistant to carbapenem. All isolates carried bla oxa-۵۱like gene. Among carbapenem resistant isolates, ۷۹.۳۸% carried bla VIM and ۱.۰۳% had bla IMP genes. Among the MBL- producing isolates, ۷ isolates were MDR, ۷۳ ones were XDR and ۵ isolates were PDR.Conclusion: This study also revealed that suceptibility to carbapenems in the population of A. baumannii isolates reduced and the bla VIM gene was the most prevalent metallo-beta-lactamase genotype among carbapenem resistant A. baumannii isolates in this area. MBL-producing A. baumannii in recent years has become a serious concern. Rapid identification and good infection control are requiered to reduce their impact.

کلیدواژه ها:

Acinetobacter baumannii ، Metallo-beta-lactamases ، Carbapenem resistance ، Polymerase Chain Reaction