The Subfamily of DEAD-Box RNA Helicases Expressionbetween Human Tes tis of Patients with Non-Obs tructiveAzoospermia and Normal Cells

سال انتشار: 1401
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 107

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شناسه ملی سند علمی:

RROYAN23_153

تاریخ نمایه سازی: 17 دی 1401

چکیده مقاله:

Background: DEAD-box RNA helicases are essential regulatorsof RNA metabolism and have been implicated in the developmentof man reproduction. These helicases cons titute a majorrecurring family of RNA-binding proteins vital for protectingthe genome.Materials and Methods: We used microarray to examine the۵۴ DEAD-box RNA helicases subfamily expression genes inthe five human tes tis sections of the normal case, azoospermicpatients and utilized in-silico databases to predict pathwaysfunctional and molecular enrichment. Current s tudies haveprovided insight into the link between genomic s tability andseveral DEAD-box RNA helicase families, including DDX۱۷,DDX۱۱, DDX۴۹, and DDX۳۱.Results: The microarray analysis of human cases with differentlevels of non-obs tructive Azoospermia revealed that expressionof DDX۱۷ was up-regulated and expression of DDX۴۹,DDX۱۱, and DDX۳۱ was down-regulated with the normal case.For this purpose, Enrichr Shiny GO databases were used forpathway enrichment analysis and gene ontology. S tRING wasapplied to predict proteins' functional and molecular interactionsand then performed to recognize the mas ter pathways.Functional enrichment analysis showed that the biological process(BP) term "Ribosome biogenesis" and "rRNA modificationin the nucleus and cytosol "was significantly overexpressed inup-regulated differentially expressed genes (DEGs). BP investigationof down-regulated DEGs highlighted "RNA binding",and "Spliceosome". Overrepresented molecular function (MF)terms in up-regulated DEGs included "double-s tranded RNAbinding" and "ubiquitin-protein protease activity". In addition,MF analysis of down-regulated DEGs showed overexpressionin "acetylation-dependent protein binding" and "Adenosine riboproteinbinding".Conclusion: Our findings sugges t that the DEAD-box RNAhelicases subfamily can help determine the pathophysiology ofnon-obs tructive Azoospermia

نویسندگان

D Hashemi Karoii

Biotechnology, Amol University of Special Modern Technologies,Amol, Iran.

H Azizi

Nanobiotechnology, Amol University of Special Modern Technologies,Amol, Iran.