Effect of L-carnitine in Freezing Extender on Motility,Viability and Lipid Peroxidation of Ram Semen

Background: Ovine semen cryopreservation is not efficientfor artificial insemination. L-carnitine (LC) has been evaluatedin cryopreservation media to improve the sperm parameters ofram after freezing-thawing process. The purpose of this s tudywas to analyze the effects of different concentrations of LC inextender for frozen-s torage of ram semen. Motility characteristics, abnormal morphology, viability and lipid peroxidation were evaluated after cryopreservation.Materials and Methods: Semen samples were collected from۵ Zell rams, twice a week, then diluted in the extenders thatcontained different concentrations of LC as follows: Tris extenderwithout LC (control), Tris containing ۱ mM (L ۱), ۲ mM(L ۲), ۴ mM (L ۴) and ۱۰ mM (L ۱۰) LC.Results: Supplementation of Tris with ۴ mM LC producedhigher significant of total motility (۴۱.۶ ± ۱.۲ %), progressivemotility (۲۲.۵ ± ۱.۴ %), viability (۴۸.۹ ± ۱.۷ %) and lower significantof lipid peroxidation (۲.۶۷ ± ۰.۱۱ nmol/ml) comparedto other groups. The lower significant of motility, progressivemotility, and viability were observed in frozen-thawed sperm inextender containing ۱۰ mM LC (۳۰.۰ ± ۱.۲ %, ۷.۵ ± ۱.۴ % and۳۱.۱ ± ۱.۷ %, respectively) compared to others. Morphologywas not affected by different concentrations of LC.Conclusion: Our results revealed that supplementation of Trisextender with ۴ mM LC significantly improved the ram spermquality after cryopreservation

کلیدواژه ها:

L-carnitine ، Ram ، Sperm Freezing