Determining the effect of the hydroalcoholic extract ofTerminalia chebula on the tachyzoite of the Toxoplasma parasite inlaboratory conditions (in vitro)

Background and Aim : Toxoplasmosis is a disease caused by Toxoplasma gondii parasite.Considering the increase in the prevalence of this disease and the increasing reports of resistanceto the common anti-toxoplasmic drugs and the possible teratogenicity of these drugs, it is necessaryto study and investigate the possibility of finding an alternative medicine, even if it is herbal, forthe treatment of toxoplasmosis. Due to this necessity, the use of hydroalcoholic extract ofTerminalia chebula as anti-bacterial, anti-fungal and anti-cancer compounds is the basis of thepresent study. The aim of the present study is to investigate the anti-toxoplasmic effects of thehydroalcoholic extract of the Terminalia chebula plant on the Toxoplasma gondii parasite in vitro.Methods : Rh strain Toxoplasma gondii parasite is obtained from the country's toxoplasmosisresearch center located in the Faculty of Health, Tehran University of Medical Sciences and isinoculated into the peritoneum of laboratory white mice until the number of parasites reaches۵۰۰.۰۰۰ per milliliter. The hydroalcoholic extract of the Terminalia chebula was prepared in serialconcentrations of ۱۰۰, ۲۰۰, ۴۰۰ and ۸۰۰ μg/ml. The effect of different concentrations at ۶۰, ۱۲۰and ۱۸۰ minutes on the parasite in ۲۴-well plates containing RPMI-۱۶۴۰ was investigated incomparison with the positive control. The results were obtained using SPSS ۲۲ software, analysisof variance, posterior LSD and repeated meager tests for data analysis.Results : The hydroalcoholic extract of Terminalia chebula in all concentrations had acceptableanti-parasitic effects against Toxoplasma gondii parasite. At a concentration of ۸۰۰ micrograms/mlof the hydroalcoholic extract of Terminalia chebula after ۶۰ minutes, better effects than thepositive control have been reported.Conclusion : According to the killing power of the hydroalcoholic extract of the Terminaliachebula on the Toxoplasma gondii parasite and its better effect than the positive controls, it can beconcluded that the above compounds can be suitable candidates for treatment after further studies.

کلیدواژه ها:

Toxoplasma gondii ، anti-toxoplasmosis ، hydroalcoholic extract ، Terminalia chebula