Effect of Cytokines Gene Expression and Serum Level of Vitamin D on the Severity of COVID-۱۹

سال انتشار: 1401
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 43

فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJMM-16-5_007

تاریخ نمایه سازی: 16 مهر 1401

چکیده مقاله:

Background and Aim: The COVID-۱۹ disease is an emerging infectious disease that appeared in December ۲۰۱۹ in Wuhan, China. An uncontrolled systemic inflammatory response is one of the primary mechanisms causing death in this disease. In this study, the expression levels of some inflammatory cytokines, vitamin D, and some hematological and biochemical parameters were compared in patients with severe COVID-۱۹ and mild types. Materials and Methods: In this cross-sectional study, ۶۰ blood samples were taken from ۳۰ severe coronavirus patients and ۳۰ mild coronavirus patients. The expression levels of cytokines such as IL (interleukin)-۶, interferon (IFN)-α, IL-۱۲, transforming growth factor (TGF) β, IL-۸ and tumor necrosis factor (TNF)-α were evaluated using Real-time PCR. A T-test was used for Statistical Analysis. Results: IL-۶, IFN-α, IL-۱۲, TGF-β, IL-۸, and TNF-α cytokines in the peripheral blood of severe patients, were positive in ۲۸/۳۰ (۹۳.۳۳%), ۲۷/۳۰ (۹۰%), ۲۴/۳۰ (۸۰%), ۲۵/۳۰ (۸۳.۳۳%), ۲۶/۳۰ (۸۶.۶۶%), and ۲۷/۳۰ (۹۰%) respectively. The positive rate of these cytokines in the mild patients were ۲۰/۳۰ (۶۶.۶۷%), ۲۱/۳۰ (۷۰%), ۱۸/۳۰ (۶۰%), ۱۷/۳۰ (۵۶.۶۷%), ۱۹/۳۰ (۶۳.۳۳%), ۱۸/۳۰ (۶۰%), respectively. There was a statistically significant difference between these two groups in terms of cytokines biomarkers. A significant difference was found between both groups in terms of the serum level of lactate dehydrogenase (LDH), the mean number of lymphocytes and neutrophils as well as the mean percentage of neutrophils/ lymphocytes ratio (NLR). Conclusion: The expression of cytokine genes and their release into the peripheral blood was increased in both severe and mild patients with COVID-۱۹. However, they were more intense in patients with severe symptoms than those with mild symptoms and can cause inflammatory and even destructive reactions. Vitamin D deficiency plays no role in causing severe COVID-۱۹ in patients without risk factors. Severe COVID-۱۹ is characterized by elevated serum levels of LDH and NLR≥۳.۴۵.

نویسندگان

Mehdi Kazempour Dizaji

Biostatistics Department, Mycobacteriology Research Center, National Research Institute of Tuberculosis and lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Hamidreza Jamaati

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Naghmeh Bahrami

Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Behrooz Farzanegan

Critical Care Quality Improvement Research Center at Shahid Modarres Hospital, Department of Anesthesiology, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mahsa Rekabi

Pediatric Respiratory Diseases Research Center, National Research Institute of Tuberculosis & Lung Diseases (NRITLD), Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mogtaba Mokhber Dezfuli

Lung transplantation Research Center, National Research Institute of Tuberculosis, and lung Diseases, Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Jalal Heshmat Nia

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammadreza Madani

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mahya Daustani

Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran

Sadegh Shirian

Department of Pathology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

Ladan Masoumi

Shefa Khatam Neuroscience Research Center, Tehran, Iran

Amir Ghaemi

Department of Virology, Pasture Institute, Tehran, Iran

Armita Narimani

Department of Cell and Molecular Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Mehran Khakbaz

Department of Cell and Molecular Biology, School of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran

Abdolreza Mohamadnia

Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Mohammad Varahram

Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

Ali Akbar Velayati

Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • Lamb LE, Bartolone SN, Ward E, Chancellor MB. Rapid Detection ...
  • Shi Z, Hu Z. A review of studies on animal ...
  • Dariushnejad H, Ghorbanzadeh V, Akbari S, Hashemzadeh P. Designing a ...
  • Abdollahi A, Mahmoudi-Aliabadi M, Mehrtash V, Jafarzadeh B, Salehi M. ...
  • De Wit E, Van Doremalen N, Falzarano D, Munster VJ. ...
  • Huang C, Wang Y, Li X, Ren L, Zhao J, ...
  • Chen N, Zhou M, Dong X, Qu J, Gong F, ...
  • Wang D, Hu B, Hu C, Zhu F, Liu X, ...
  • Hui RK, Zeng F, Chan CM, Yuen K, Peiris JS, ...
  • Peiris M, Guan Y, Yuen K. Severe acute respiratory syndrome: ...
  • Williams AE, Chambers RC. The mercurial nature of neutrophils: still ...
  • Min C-K, Cheon S, Ha N-Y, Sohn KM, Kim Y, ...
  • To KK-W, Tsang OT-Y, Yip CC-Y, Chan K-H, Wu T-C, ...
  • Heydar H, Mansouri K, Norooznezhad M, Norooznezhad F, Mohamadnia A, ...
  • Karimi S, Bahrami N, Sharifi K, Daustany M, Baghbani-Arani F, ...
  • Bahrami N, Gholami M, Jamaati HR, Mohamadnia A, Dargahi H, ...
  • Ghadimi K, Bahrami N, Fathi M, Farzanegan B, Naji T, ...
  • Farzanegan B, Bahrami N, Birjandi B, Khosravi A, Nasab AF, ...
  • Bolandparva F, Nasab MSH, Mohamadnia A, Garajei A, Nasab AF, ...
  • Ozaki K, Leonard WJ. Cytokine and Cytokine Receptor Pleiotropy and ...
  • Dinarello CA. Historical insights into cytokines. Eur J Immunol. ۲۰۰۷;۳۷(S۱):S۳۴-S۴۵ ...
  • Scarpioni R, Ricardi M, Albertazzi V. Secondary amyloidosis in autoinflammatory ...
  • Opal SM, DePalo VA. Anti-inflammatory cytokines. Chest. ۲۰۰۰;۱۱۷(۴):۱۱۶۲-۷۲ ...
  • Zinchuk A, Holubovska O, Shkurba A, Hrytsko R, Vorozhbyt O, ...
  • Arend WP. The mode of action of cytokine inhibitors. J ...
  • Shaikh PZ. Cytokines & their physiologic and pharmacologic functions in ...
  • Zhang Y, Li J, Zhan Y, Wu L, Yu X, ...
  • De Jong MD, Simmons CP, Thanh TT, Hien VM, Smith ...
  • Cillóniz C, Shinya K, Peng X, Korth MJ, Proll SC, ...
  • Tavakoli-Ardakani M, Abbaspour H, Nasab AF, Meibodi AM, Kheradmand A. ...
  • Channappanavar R, Perlman S. Pathogenic human coronavirus infections: causes and ...
  • Teijaro JR. Cytokine storms in infectious diseases. Semin Immunopathol; ۲۰۱۷;۳۹(۵):۵۰۱-۳. ...
  • Xi-zhi JG, Thomas PG. New fronts emerge in the influenza ...
  • Channappanavar R, Fehr AR, Vijay R, Mack M, Zhao J, ...
  • Shaw AC, Goldstein DR, Montgomery RR. Age-dependent dysregulation of innate ...
  • Davidson S, Maini MK, Wack A. Disease-Promoting Effects of Type ...
  • Li X, Geng M, Peng Y, Meng L, Lu S. ...
  • Cheng OZ, Palaniyar N. NET balancing: a problem in inflammatory ...
  • Wu F, Zhao S, Yu B, Chen YM, Wang W, ...
  • Alosaimi B, Hamed ME, Naeem A, Alsharef AA, AlQahtani SY, ...
  • Lau SK, Lau CC, Chan K-H, Li CP, Chen H, ...
  • نمایش کامل مراجع