In-vitro antioxidant therapy and improvement of sperm parameters and quality in asthenoteratozoospermia patients

Backgrounds: Spermatozoa are very vulnerable to oxidative stress caused by an imbalancebetween ROS and antioxidants in the male reproductive system. Despite clinical studiesreporting positive effects of oral antioxidants on sperm physiology and fertility, only a fewstudies have been performed to evaluate their effects on sperm in vitro. In sperm cells, CoenzymeQ۱۰ is the most important intracellular antioxidant, so that motility and other energydependentprocesses depend on the amount of access to this antioxidant. The main purpose ofthis study was to evaluate the effect of adding CoQ۱۰ to a sperm preparation medium on thefunctional parameters.Materials and Methods: Semen samples, obtained from ۳۰ oligoasthenospermia patients wereenrolled in this study.Spermatozoa were washed with Ham’s F-۱۰ media containing ۱۰% human serum albumin andthe antioxidant, and then the motility, Viability and DNA fragmentation of the spermatozoa wereassessed.Results: The addition of this antioxidant to the sperm preparation medium significantlyimproved the motility and viability of the spermatozoa compared with the control group.Moreover, Co- Q۱۰ also decreased the DNA fragmentation rate of the spermatozoa. Main resultsdeclared that in specimen, total sperm motility is maintained by in vitro treatment with CoQ۱۰whereas a significant decrease of these parameters occurs in parallel samples incubated inmedium alone.Conclusion: Supplementing sperm preparation medium with Co-Q۱۰ improved the overallfunctional parameters of the spermatozoa.

کلیدواژه ها:

Sperm parameters ، Antioxidant therapy ، DNA fragmentation ، Asthenoteratozoospermia