TXNDC۸ mRNA expression Evaluation in semen of infertile men

Backgrounds: Azoospermia is one of the most severe types of infertility and one of the mostimportant eras of research is the possibility of sperm retrieval. Thyorodoxin gene familyespecially TXNDC۸ has been recently highlighted in male infertility. The corresponding proteinnot only acts as a specific sperm oxidoreductase agent but is also involved in the formation ofdisulfide bonds during sperm nuclei maturation. In the present study, the mRNA expression levelof the TXNDC۸ gene would be assessed in semen to evaluate its potency as a marker of spermretrieval.Materials and Methods: Twenty non-obstructive azoospermic men (NOA, as a patient group)and an equal number of obstructive azoospermic (OA, control group) men would enter the study.RNA extraction would be performed and cDNA would be synthesized with the help of randomhexamers. mRNA expression would be performed by qRT-PCR and the Livak statistical methodwould be used to compare the gene expression between comparing groups.Results: Our primary data analysis on microarray Gene Ontology Omnibus (GSE۴۵۸۸۵) showeddownregulation of the TXNDC۸ gene in NOA men compared to OA control individuals. Theobserved downregulation was completely significant (LogFC=-۱.۰۳۴۷۰۶, p=۰.۰۱۲۸۸۶<۰.۰۵). Itis proposed that similar results would be observed in our lab experimentation.Conclusion: Because TXNDC۸ mRNA expression is related to sperm parameters and itschromatin structure, it could be a suitable molecular marker for predicting sperm retrieval inNOA patients.

کلیدواژه ها:

TXNDC۸ ، Semen ، Azoospermia ، thyroxine