Study of nettle extract and shigatoxin effects on the inflammation-wound healing process in mouse

Backgrounds: The use of modern treatment strategies in wound healing and control ofinflammatory processes is one of the essential needs of the medical and human community.Understanding the biological effects and cellular and molecular basis of drugs of plant andmicrobial origin will enable the effective and purposeful use of these products.Materials and Methods: In this study, after creating superficial wounds on the skin of mice inboth control and treatment groups, a comparative study of the simultaneous effect of nettleextract and Shiga toxin isolated from Shigella dysenteriae on the wounds in the treatment groupcompared with the control group was performed. TGFB gene expression was examined as anindicator of inflammation and wound healing rate was studied after comparing the woundhealing process and quantifying it using Image J software.Results: The results showed a significant decrease in TGFB gene expression in the treatmentgroup (p≤۰.۰۵, FC=۰.۳) compared to the control group and the curve of changes in woundhealing rate and reduction of injury area showed an acceptable slope.Conclusion: It seems that nettle extract along with Shiga toxin can be effective in wound healingrate without causing a significant inflammatory response, by modulating the inflammatoryresponse and increasing blood flow in the wound area.

کلیدواژه ها:

Wound healing ، Nettle extract ، Shigatoxin ، Gene expression