The effect of aerobic training and probiotic intake on gene ICAM–۱ expression in rats with nonalcoholic fatty liver

Background: Cell adhesion molecules mediate leukocyte responses to inflammation. The purpose of study was to the effect of aerobic training and probiotic intake on ICAM–۱ in rats with fatty liver. Materials and Methods: In this experimental study, ۳۲ rats (۲۰۰-۲۵۰ gr) were randomly divided into four groups (healthy control, steatosis, steatosis + probiotic, steatosis + probiotic + training). To create a fatty liver model, tetracycline at a dose of ۱۰۰ mg / kg in a volume of ۱.۵ cc was gavaged daily for two weeks. The training program includes ۸ weeks and ۵ sessions per week, in the initial week with a speed of ۱۸ meters per minute, time started ۱۰ minutes and every week quickly, ۱-۲ meters per minute and time was added to ۱۰ minutes. Supplemental groups received ۱۰۹ CFU / ml of Lactobacillus ramensus by gavage daily for ۸ weeks and ۵ days per week. Liver tissue samples were taken to examine the expression of ICAM-۱ gene. ۱۰ ml of blood was collected from the hearts of rats for ALP testing. The data was analyzed using one-way analysis of variance (ANOVA) p≥۰/۰۵. Results: The results showed that the aerobic training along with probiotic consumption significantly reduced the expression gene of ICAM-۱ and ALP in rats with non-alcoholic fatty liver. Conclusion: It seems aerobic training combined with probiotic consumption improved the expression gene of ICAM-۱ and ALP enzyme in the studied samples which can be effective in treating patients with fatty liver disease.

کلیدواژه ها:

Non-alcoholic fatty liver ، Probiotic ، ICAM-۱ ، ALP