Dysregulation of LINC۰۱۹۸۵ lncRNA leads to the abnormal exocytosis by low expression of MMRN۱ in the thyroid cancer patients: an integrated computational biology approach

Genomic characterization and evolutionary histories of both primary and metastatic cancer development havebeen made possible by high-throughput and multi-omics technologies. These tools, which give insight intocancer cells' genome, transcriptome, metabolome, and proteome, confirm our grasp of systems biology-levelapproaches to cancer. This study performed a high-throughput microarray data analysis to find novelregulatory genes in the thyroid cancer samples, using a systems biology approach. Furthermore, the RNAinteractionand functional enrichment analysis was performed to demonstrate the precise regulatorymechanism of the coding and non-coding RNAs found in the experiment. The GSE۳۳۶۳۰ microarray datasetwas analyzed by R Studio (۴.۰.۲). Normalization of raw data was performed by affy package. Differentialexpression (DE) and statistical analyses were performed by limma package. RNA interaction and functionalenrichment analyses (Pathway and Gene Ontology (GO)) was performed by Enrichr online software.MMRN۱ has a significantly low expression in the thyroid cancer patients, compared to control (logFC: -۴.۲۰۵, adj. P. Value < ۰.۰۰۰۱). LINC۰۰۴۷۷ and LINC۰۱۹۸۵ lncRNAs have a significant co-expression withthe NMRN۱ mRNA (adjusted p-value: ۰.۰۰۵). Based on literature mining, LINC۰۰۴۷۷ isoforms can have asignificant role in developing gastric cancer (by down-regulation). In this study, we demonstrate the possibleregulatory role of the two mentioned lncRNAs in developing thyroid cancer for the first time by disturbanceof the regulation of exocytosis (GO:۰۰۴۵۰۵۵) in the secretory granule lumen (GO:۰۰۳۴۷۷۴). LINC۰۰۴۷۷and LINC۰۱۹۸۵ lncRNAs are the two novel non-coding RNAs that can regulate the expression of MMRN۱.Dysregulation of the two mentioned RNAs can lead to the abnormality in the expression of MMRN۱ anddisturb the regular exocytosis mechanism in the secretory granule lumen in thyroid cancer patients.

کلیدواژه ها:

Systems biology ، High-throughput data ، Microarray ، MMRN۱ ، RNA interaction