Bioinformatics evaluation of hsa-miR-۴۴۹۶ related to a single nucleotide polymorphism (rs۱۰۳۱۳۰۵۳۳۰) of GSTP۱ gene in gastric cancer

Introduction: Gastric cancer remains one of the most common and deadly cancers worldwide. Helicobacterpylori infection is the major risk factor associated with the development of gastric cancer (GC). The transitionfrom normal mucosa to non-atrophic gastritis, triggered primarily by H. pylori infection, initiatesprecancerous lesions which may then progress to atrophic gastritis and intestinal metaplasia. GSTP۱ gene islocated position on choromosome۱۱q۱۳.۲. Different studies indicated that this gene is associated with gastriccancer. Based on accomplished studies, expression of this gene may be a prognostic indicator for several typeof cancer. Single nucleotide polymorphisms (SNPs) are consisting genetic marker related to many of thegenetic disease. microRNAs are counted as bio- markers with regulation and control of genes expression atlevel of mRNA. Given the importance of SNPs and microRNAs as cancer biomarkers that influence theexpression of genes, this study aimed to investigate association between hsa-miR-۴۴۹۶ and a singlenucleotide (rs۱۰۳۱۳۰۵۳۳۰) of GSTP۱ gene in patients suffering from gastric cancer.Methods: In the present study, databases such as NCBI, miRNASNP-V۳, miRWalk, PhenomiR, miRBaseand David were used for in silico data analyses.Results: Our bioinformatics analysis indicated that rs۱۰۳۱۳۰۵۳۳۰ is associated with the conversion of C toT allele in GSTP۱ gene, which may affect the performance and binding of miRNAs in this region. In thisregard, results of this study also revealed that hsa-miR-۴۴۹۶ can bind to the ۳'-UTR transcript of the GSTP۱gene.Conclusion: Altogether, this study identifies rs۱۰۳۱۳۰۵۳۳۰ and hsa-miR-۴۴۹۶ as important biomarkers thatare associated with GSTP۱ gene and gastric cancer. Also, it suggests that the formation of the T allele inrs۱۰۳۱۳۰۵۳۳۰ affects the binding of hsa-miR-۴۴۹۶ to the GSTP۱ gene.

کلیدواژه ها:

Single nucleotide polymorphism ، rs۱۰۳۱۳۰۵۳۳۰ ، GSTP۱ gene ، hsa-miR-۴۴۹۶ ، Gastric cancer