Investigation of renal tubular cells gene expression during acute kidney injury induced by Cisplatin

Acute kidney injury (AKI) is defined by a sudden loss of excretory kidney function. (۱) AKI is part of a rangeof conditions summarized as acute kidney diseases and disorders (AKD), in which slow deterioration ofkidney function or persistent kidney dysfunction is associated with an irreversible loss of kidney cells andnephrons, which can lead to chronic kidney disease (CKD). (۲, ۳, ۴) As a consequence, finding signalingpathways related to the proximal tubule injury by bioinformatics approaches is essential. In this study wefocused on gene expression profiling in damaged Tubular cells and investigated signaling pathwaysassociated with this matter. At first, we selected suitable studies from GEO database (GSE۸۵۹۵۷). Thisdataset consists of three groups of injured PTCs with four difference times (D۳, D۵, D۸ and D۲۶) andanalyzed with GEO۲R tool. Then uploaded the up and down regulated genes to VENNY version ۲.۱.۰ tooland selected processed in common genes between up/down regulated groups to examine the signalingpathways in the DAVID database and the KEGG library then loaded the genes involved in biological process,cell component and molecular function. Finally, we used STRING database to select protein interaction.Results showed that ۱۲۷ genes up regulated and ۱۲۸ genes were down regulated. Among those up regulatedgenes most of them were expressed in p۵۳ signaling pathway, Ribosome, TNF signaling pathway, HTLV-Iinfection in other hand metabolic pathways, Citrate cycle (TCA cycle) and Carbon metabolism were observedin down regulated genes. Mdm۲ ،Cdkn۱a ،Egr۱ ،Fos ،Rps۷ ،Atf۳ genes were up regulated and the resultsalso obtained that Adk, Pdha۱، Sdha ،Sdhd ،Uqcrc۲ were down regulated. By this analysis and results weobserved the genes that identified in tubular cells damages that highly related to ribosome, metabolicpathways.

کلیدواژه ها:

Acute kidney disease ، Proximal tubule cell ، Microarray analysis ، gene expression profile