The Effect of Prolonged Incubation of Sperm at Testis Temperature versus Room Temperature on Semen Parameters- An Experimental Study

Background & Objective: The effect of storage time and temperature on the prepared semen sample was evaluated, but the optimal condition is unclear. The aim of this study was to assess the effect of long-term incubation of prepared sperm at testicular temperature versus room temperature on semen parameters and DNA fragmentation index (DFI). Materials & Methods: Sperm samples were collected from ۴۰ patients between ۲۰۱۹ and ۲۰۲۰. Each sample was separated into two parts and underwent a non-direct swim-up method. One group was placed in a ۳۵°C incubator, and the other group was kept at room temperature (۲۶°C) in the dark. Both groups were evaluated at intervals of ۴۵ minutes, ۲۴ hours and ۴۸ hours after sampling in terms of sperm concentration, motility, morphology, and DFI. Student t-test and repeated measures analysis of variance were used. Results: Sperm count (P=۰.۰۰۷) and motility (P<۰.۰۰۱) at ۲۶°C in three-time intervals of ۴۵ minutes, ۲۴ hours and ۴۸ hours were significantly higher than ۳۵°C. The proportion of normal morphology spermatozoa at ۲۶ and ۳۵°C at ۴۵ min, ۲۴ h, and ۴۸ h did not show a significant difference (P=۰.۰۸). DFI at ۲۶°C in three-time intervals was significantly lower than ۳۵°C (P=۰.۰۰۸). Conclusion: The results of this study indicated that when the prepared sperm samples are incubated for ۲۴ h at ۲۶°C compared to ۳۵°C, they show significantly better quality and good quality of sperm can be retained for several hours if stored at room temperature.

کلیدواژه ها:

Assisted reproductive techniques ، DNA fragmentation ، Insemination ، Spermatozoa ، Temperature