Induction of neural stem cell proliferation by iron oxide nanoparticles and magnetic field and Ki۶۷ gene expressionin rat hippocampus after ischemia/reperfusion

Background and aims: Ischemic stroke is considered as the second leading cause of death in the world and yet one of the causes ofdisability in adults. The present study aimed to evaluate the effects of iron oxide nanoparticles and the magnetic field on neural stemcells proliferation after ischemia/reperfusion in the rat model.Methods: This experimental study was conducted on a total of ۵۰ male Wistar rats aged ۶-۷ weeks and weight of ۲۲۰-۲۵۰ g, whichwere divided into sham (i.e., ischemia-reperfusion model), control, iron oxide nanoparticles treated-, magnetic field exposed-, andsimultaneously iron oxide nanoparticles and magnetic field exposed- groups. The brain ischemia/reperfusion was performed for ۲۰minutes by blocking the animal carotid arteries. In addition, neural stem cell proliferation was evaluated in the hippocampus of the ۵groups after ۴ days by bromodeoxyuridine (BrdU) staining method. Then, the expression of Ki۶۷ gene involved in the cell proliferationwas quantitatively studied among the ۵ groups by the quantitative real-time polymerase chain reaction (qRT-PCR).Results: The results of BrdU staining revealed that iron oxide nanoparticles and the magnetic field separately increased cell proliferationafter ischemia/reperfusion after ۴ days in the hippocampus. However, simultaneous treatment with nanoparticles and magnetic fieldfailed to show a significant difference compared to the sham group for ۴ days. Conversely, the expression of Ki۶۷ gene increasedsignificantly in the group treated with iron oxide nanoparticles or the group exposed to magnetic field compared to the ischemiareperfusionmodel.Conclusion: In general, iron oxide nanoparticles and magnetic field can separately be regarded as ۲ effective methods for increasingthe neural stem cell proliferation after ischemia/reperfusion.

کلیدواژه ها:

Iron oxide nanoparticles ، Ischemia/reperfusion ، Ki۶۷ ، Magnetic field ، Q-RT-PCR