Anti bacterial effects of Saponair officinalis extracts (saponin) against Motile salmonella SP. (a farm Isolate)

Now a days antibiotic residues in crude or cooked foods are the most ant nutrient materials of foods, they not only decrease the nutritional value of foods but also caused bacterial resistances, hepattonephric damages and sometimes they are carcinogen or teratogens .if the organoleptic characters of such food be afraid.In other hand using antibiotics for disease control in broilers and or breeders (egg layers) can not be forbidden, but it could manages for preparing a safe food or it is possible present new natural antibiotics what we mean as natural drugs like herbal drugs, nonmaterial and so on., Ancients used Saponaire flowers and risomes for washing the hair and body skin in bathroom also diuretic and blood refining characters.In current study using Saponaire Of. (related to Caryophllacaea familly) hydro alcoholic extracts for invitro inhibition growth of an Isolated fatal motile Salmonella Sp.were reported .The saponaria extracts which is called as saponin, therefore after isolation and identification of isolated Motile Salmonella Sp. using common bacterologic and biochemical tests, the disc diffusion method was used for avaluation the antibacterial effects of saponaire extracts in comparison to some routine antibiotics .Hydroalcohoic extracts of saponaire officinalis prepared and save in ۴C dark tube Some filter papers punched, sterilized and used as disk for preparation the saponine disks as ۱۰ μ Li ,۱۵ μ Li and ۲۰ μ Li and incubated ۱۵ hrs at ۳۷ C for drying.The Isolated E-coli were propagated in Muller hintone broth and incubated at ۲۵ C for ۶ hrs adjusted to Mc Farland standard and cultured on Muller hintone agar for antibiogram tests, meanwhile ۳ current .antibiotic disc included Oxytetracycline(Oxy), Danofloxacin(Dfx) and Fosbac(Fos) for compartment in ۳ times, in each exam, cultured test plate incubated overnight in ۳۷C and immediately the zone of inhibition diameter were measured .Based on the results the zone of inhibition for ۱۰ μ Li ,۱۵ μ Li and ۲۰ μ L of Saponiare disks were ۱۶ mm,۱۸ m and ۱۹ mm respectively, and the zone of inhiboition diameter for antibiotic disks of Fos ,Oxy and Dfx were ۱۵ mm, ۰ mm and ۱۴ mm respectively.

کلیدواژه ها:

Antibiogram ، Salmonella ، Motile ، Saponin ،