Relationship Between FGFR۴ Gene rs۳۵۱۸۵۵ G/A Polymorphism and the Risk of Lung Cancer in the Northern Provinces

Lung cancer is the leading cause of cancer death in men and the second leading cause of cancer death in women worldwide. FGFR is involved in a variety of cellular processes including angiogenesis, wound healing, tissue repair, and tumorigenesis. Recently, a common polymorphism in the transmembrane domain of the FGFR۴ gene, Gly۳۸۸Arg, has been reported to correlate with alteration of cell migration in vitro and with disease progression and/or survival in breast, colon, prostate and lung cancer. To evaluate the prognostic significance of the FGFR۴ Gly۳۸۸Arg polymorphism in lung cancer, we analyzed a case-control study of ۱۱۰ lung cancer patients and ۹۰ healthy control. Genomic DNA from whole-blood specimens was extracted using Salting-out method. Quality of DNA was evaluated by electrophoresis. To determine the distribution of FGFR۴ Arg۳۸۸ and FGFR۴ Gly۳۸۸ alleles in lung carcinoma patients, used RFLP-PCR. in this study demonstrated that there was no relationship between polymorphism of FGFR۴ Gly۳۸۸Arg gene and lung cancer. Also, no significant relationship was observed between this polymorphism and clinical and pathological features of patients. It is suggested the large case-control studies are needed to detect genetic determinants affecting patients’ prognosis, with the promise of targeting these putative genetic determinants to provide new therapeutic tools for patients with lung cancer.

کلیدواژه ها:

Lung cancer ، single nucleotide polymorphism ، FGFR۴ ، RFLP-PCR