Statistical optimization of the biosurfactant production from Lactobacillus brevis isolated from dairy products

Background and Aim : Biosurfactants are well known as one of the adhesion inhibitors to prevent pathogens from attaching to the adhesion on epithelial cells of urogenital and intestinal tracts and can be used by humans despite being a probiotic source. The main purpose of this study was to optimization of the biosurfactant production from Lactobacillus brevis isolated from dairy products. Methods : In this study, L. brevis was isolated from dairy products during screening of ۷۰ isolates using MRS agar. The optimal cultural conditions for biosurfactant production obtained with Response Surface Methodology experimental design (Central Composite Design; CCD). ۲۰ experimental combinations were designed to investigate three independent factors including Oil content, Yeast concentration, and Glucose concentration at five various levels with six repetitions at the central point.Results : The results of optimization of the biosurfactant production showed the biosurfactant Oil Spreading ranged from ۲۸ mm to ۹۹ mm, and the experiments of ۹ and ۷ gave the minimum and maximum Spreading, respectively. The quadratic model was summarized after removing the insignificant coefficients. According to the predicted model, the maximal biosurfactant production was achieved at ۲.۷۵% of glucose, ۳.۶۲۵% of oil and ۴.۲۵% of yeast concentration.Conclusion : The biosurfactant of Lactobacillus brevis is expected to consider an appropriate candidate can be applied to adhesion inhibitors, to inhibit human pathogenic biofilm producers.

کلیدواژه ها:

biosurfactant ، Lactobacillus ، probiotic ، optimization