Identification of flavonoid constituents in Teucrium melissoides, T. parviflorum, T. oliverianum and T. scordium (Lamiaceae) from Zagros region, Iran

The genus Teucrium L. belonging to Lamiaceae family and Ajugoideae sub-family with numerous medicinal properties, has been considered in pharmaceutical science and traditional medicine. The aim of this research is to determine the flavonoid compounds and detect chemical markers in four Teucrium species belonging to the center, west, south and southwest of Zagros regions. The flavonoid of dried leaves (۱۰ gr) was isolated using pure MeOH and rotary evaporator at ۷۰°C. In order to purify flavonoids and prepare several fractions, column chromatography (sephadex LH۲۰) with ۱۰۰ mL CHCl۳-MeOH ۶۰:۴۰ was applied. In addition, the identification process of flavonoid compounds was achieved using High Performance Liquid Chromatography Mass Spectrometry technique. The mass spectrometry (MS) was arranged in the range of m/z ۲۶۷-۷۸۵ using negative ionization mode and full scanning. In total, five fractions were investigated in MS/MS process. The results of this research showed ۹۳ flavonoid compounds. The highest number of flavonoid compounds belonged to T. scordium L. (۴۰ compounds). Some tannins and hydroxycinnamic acid derivatives were also recognized in low quantities. The most important flavonoid compounds in four species included dihydrorotenone (isoflavone), daidzein (iosoflavone), isorhamnetin (flavonol), diosmin (flavone), myricetin (flavone), artocarpin (flavone), isosakuranetin (flavanone), and methoxychalcone (chalcone) derivatives. In total, eight chemical markers were identified for each species including tectochrysin-glucoside, biochanin-malonylglucoside (T. melissoides Boiss. ex Hausskn. ex Boiss.), kumatakenin, dihydroxy-trimethoxychalcone (T. olivieranum Ging. ex Benth.), aspalathin, robinetin trimethyl ether (T. parviflorum Schreb.), chrysoeriol-diglucoside, and irigenin (T. scordium). Finally, High Performance Liquid Chromatography Mass Spectrometry is a powerful technique to exhibit different flavonoid compounds and chemical markers for Teucrium species. The evidence of this research is useful for chemotaxonomic, pharmaceutical and therapeutic purposes.

کلیدواژه ها:

Liquid chromatography ، spectrometry ، phytochemistry