Association of the MIAT rs1894720 Polymorphism with Ischemic Stroke Risk and MIAT Expression Levels in Blood after an Ischemic Stroke

Myocardial infarction associated transcript (MIAT) is a new disease-related lncRNA characterized by an improper expression in different ailments, such as ischemic stroke (IS), schizophrenia, diabetic complications, cancers, myocardial infarction, and cataracts. It is hypothesized that the MIAT rs1894720 polymorphism is correlated with ischemic stroke risk and MIAT expression rate. We studied 116 Iranian patients with clinically definite acute IS and 116 ethnicity-, age-, and sex-matched controls. The tetra-primer ARMS-PCR method was applied for DNA genotyping. The susceptibility of IS and MIAT rs1894720 polymorphism sites was analyzed. The MIAT expression in the blood was evaluated after RNA extraction, cDNA synthesis, and real-time PCR. We used the receiver operating characteristic (ROC) curve to evaluate the diagnosis and prognosis of IS. The mean age of IS patients was 65.90±14.44 years. The polymorphism of MIAT rs1894720 was related to IS susceptibility in the recessive model (OR=8.51, 95% CI=1.04-69.23, P=0.01). Furthermore, the co-dominant (OR=0.24, 95% CI=0.07-0.76, P=0.009 for GT genotype), dominant (OR=0.26, 95% CI=0.08-0.81, P=0.01),and over-dominant (OR=0.19, 95% CI=0.07-0.52, P=0.0005) models of the rs1894720 were negatively associated with the risk of ischemic stroke. There was no correlation between rs1894720 polymorphism and MIAT expression levels. We, also, detected a significant up-regulation of MIAT gene expression in IS stroke patients in comparison to the control group. ROC curves showed that MIAT might serve as a biomarker for diagnosing IS patients. The rs1894720 polymorphism of the MIAT is correlated with the risk of IS but not with MIAT expression levels. In addition, the upregulation of blood-derived MIAT can be considered a diagnostic biomarker of IS.

کلیدواژه های Association of the MIAT rs1894720 Polymorphism with Ischemic Stroke Risk and MIAT Expression Levels in Blood after an Ischemic Stroke:

Stroke ، Long Non-Coding RNA ، Polymorphism ، Biomarkers ، Real-Time PCR